Motivation is the driving force which stimulates an individual to take action and sustains that behaviour. In the initial stage, only money was considered as a factor of motivation but there are many other factors also which motivates the individuals. The importance of these factors of motivation varies from person to person. Some people prefer financial factors while some people prefer non-financial factor. Present research aimed at identifying preference of factors affecting motivation of employees working in various Indian Enterprises and impact of demographic factors on the factors of motivation. Study was conducted with the help of questionnaire for this research. The questionnaire consisted of two parts: Part (A) and Part (B). The questionnaire was prepared on the basis of ten factors of motivation, Participants were asked to give the ranking to the factors of motivation on the basis of their importance. Relationship between motivational factors and demographic variables were established with the help of T test and ANOVA. The motivating factors were taken with the help of literature review. This research is supposed to provide useful directions to managers and other policy makers in developing employee motivation policies.